This bill would prohibit federal funding to any organization that allows individuals to use single-sex facilities like restrooms, locker rooms, or changing rooms that do not match their biological sex. The legislation applies to private entities, non-profit organizations, and state and local government agencies that receive any federal funds. It defines biological sex based on reproductive system characteristics and establishes broad penalties by cutting off all federal funding to any entity that violates the restriction. The bill includes narrow exceptions for emergency medical personnel responding to medical emergencies and law enforcement officers actively pursuing suspects or conducting investigations. No specific funding amounts or implementation timeline are included in the legislation.