This bill establishes federal legal definitions of sex, male, and female based on biological reproductive characteristics. Specifically, it defines "male" as a person with a reproductive system that produces sperm and "female" as a person with a reproductive system that produces eggs, with sex determined at conception and considered immutable. The legislation also states that "gender identity"—described as an internal sense of self disconnected from biological reality—will not be recognized by the federal government as a replacement for sex in the interpretation of federal law. The bill applies these definitions across all federal agencies and acts of Congress, affecting how the government interprets and implements existing laws. The legislation contains no funding provisions or implementation timeline, as it is purely definitional in nature.