| Bloomer Lakeview INC | Related tax-exempt org | Low income housing | WI | 391450617 |
| Charterhouse INC | Related tax-exempt org | Retirement living center | MN | 411405254 |
| MAYO Clinic | Related tax-exempt org | Clinic | MN | 416011702 |
| MAYO Clinic Ambulance | Related tax-exempt org | Ambulance service | MN | 411917516 |
| Luther Lakeside Apartments INC | Related tax-exempt org | Low income housing for elderly | WI | 391409024 |
| MAYO Clinic Arizona | Related tax-exempt org | Hospital and clinic | AZ | 860800150 |
| MAYO Clinic Florida (a Nonprofit Corporation) | Related tax-exempt org | Hospital | FL | 590714831 |
| MAYO Clinic Hospital -- Rochester | Related tax-exempt org | Hospital | MN | 410944601 |
| MAYO Clinic Jacksonville | Related tax-exempt org | Patient care - clinic | FL | 593337028 |
| MAYO Foundation for Medical Education and Research | Related tax-exempt org | Charitable, educational & scientific activities | MN | 411506440 |
| MCHS--Austin Foundation | Related tax-exempt org | Fundraising foundation | MN | 300107471 |
| MCHS--Fairmont | Related tax-exempt org | Hospital and clinic | MN | 410760836 |
| MCHS--Franciscan Medical Center INC | Related tax-exempt org | Hospital and clinic | WI | 390806374 |
| MCHS--LAKE City | Related tax-exempt org | Hospital | MN | 411906820 |
| MCHS--Northwest Wisconsin Region INC | Related tax-exempt org | Hospital | WI | 390813418 |
| MCHS--Southeast Minnesota Region | Related tax-exempt org | Hospital and clinic | MN | 411404075 |
| MCHS--Southwest Minnesota Region | Related tax-exempt org | Hospital and clinic | MN | 411236756 |
| MCHS--ST James | Related tax-exempt org | Hospital and clinic | MN | 410797368 |
| Latigo Petroleum LLC | Related partnership | OIL & gas exploration | DE | 364767494 |
| MAYO Clinic Healthcare LLP | Related partnership | Healthcare | — | — |
| MAYO Clinic Support Services Texas | Related taxable corporation/trust | Health services | TX | 471751102 |
| MAYO Clinic UK LTD | Related taxable corporation/trust | Investment activities | — | — |
| MAYO Holding Company | Related taxable corporation/trust | Holding company | MN | 411578020 |
| MAYO Insurance Company LTD | Related taxable corporation/trust | Self insurance POOL | — | — |
| Resoundant INC | Related taxable corporation/trust | Manufacturing medical device component | MN | 461661978 |