| 11101 Johns Hopkins Road Business Trust | Disregarded entity | Holds title to real estate | MD | 520595111 |
| 2733 North Charles LLC | Disregarded entity | Holds title to real estate | MD | 520595110 |
| Johns Hopkins Applied Physics Laboratory LLC | Disregarded entity | Research | MD | 520595111 |
| Charles & Blackstone Apartments LLC | Disregarded entity | Holds title to real estate | MD | 521990969 |
| Frederick Neonatal Services LLC | Disregarded entity | Clinical services | MD | 520595110 |
| JHU Clinical Billing LLC | Disregarded entity | Billing services | MD | 943438474 |
| JHU-Charles Commons LLC | Disregarded entity | Holds title to real estate | MD | 520595110 |
| Johns Hopkins Seton Properties LLC | Disregarded entity | Holds title to real estate | MD | 520595110 |
| The Johns Hopkins University (usa) Foundation Limited | Disregarded entity | Promotes education | — | 520595110 |
| MT Washington Campus North Business Trust | Disregarded entity | Business trust - real estate and related services | MD | 416517144 |
| MT Washington Campus South Business Trust | Disregarded entity | Business trust - real estate and related services | MD | 516539776 |
| Stafford Apartments LLC | Disregarded entity | Holds title to real estate | MD | 520595110 |
| 3111 North Charles Investment LLC | Disregarded entity | Holds title to real estate | MD | 830661805 |
| Johns Hopkins Global II LLC | Disregarded entity | Research and international education | MD | 831519934 |
| Hackerman Charitable Holdings LLC | Disregarded entity | Investment | DE | 520595110 |
| 63019 Holdings LLC | Disregarded entity | Investment | DE | 833939444 |
| 1876 Holdings LLC | Disregarded entity | Investment | MD | 520595110 |
| The JHU 2018 BLK Trust | Disregarded entity | Investment | CA | 836584875 |
| Evergreen House Foundation INC | Related tax-exempt org | Support cultural/EDUC facility | MD | 520627782 |
| Johns Hopkins University Center for Communication Programs (tz) Limited | Related tax-exempt org | Social awareness and communications for public health | — | — |
| Johns Hopkins Bangladesh Limited | Related tax-exempt org | Communications programs for public health | — | — |
| Johns Hopkins Pediatrics at Home INC | Related tax-exempt org | Home health services | MD | 521803773 |
| Johns Hopkins Pediatrics at Home INC | Related tax-exempt org | Home health services | MD | 521803773 |
| Johns Hopkins Public Health in Nigeria Initiative | Related tax-exempt org | Communications programs for public health | — | — |
| Johns Hopkins University HONG KONG LTD | Related tax-exempt org | Education | — | — |